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Reaper-induced apoptosis in a vertebrate system

E K Evans¹, T Kuwana, S L Strum

¹Department of Pharmacology and Cancer Biology, Duke University Medical Center, Box 3686, C366 LSRC, Research Drive, Durham, NC 27710, USA.

The EMBO Journal

|February 21, 1998

Summary

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The reaper protein triggers apoptosis in vertebrates, utilizing mitochondria and cytosolic factors. This suggests conserved components of the cell death pathway across species.

Area of Science:

Cell Biology
Molecular Biology
Developmental Biology

Background:

The reaper protein regulates apoptosis in Drosophila.
Reaper's function and required components in vertebrates remain undefined.

Purpose of the Study:

To investigate reaper's apoptotic function in a vertebrate system.
To identify cellular components necessary for reaper-induced apoptosis.

Main Methods:

In vitro apoptotic reconstitution system using Xenopus egg extracts.
Mitochondrial enrichment and analysis of cytochrome c release.
Investigation of Bcl-2 antagonism.

Main Results:

Reaper induces rapid apoptosis in vitro.

Related Experiment Videos

A mitochondrial fraction and cytosolic factors are essential for reaper's action.

Reaper triggers mitochondrial cytochrome c release.

Bcl-2 can antagonize reaper-induced apoptosis, but high reaper levels overcome this inhibition.

Conclusions:

Reaper can function in a vertebrate context.
Cellular factors mediating reaper's apoptotic activity are conserved.
Mitochondria play a crucial role in reaper-mediated cell death.