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Updated: Jul 7, 2026

Measurement of Cellular Chemotaxis with ECIS/Taxis

Measurement of Cellular Chemotaxis with ECIS/Taxis

Published on: April 1, 2012

Chemotaxis assays for eukaryotic cells.

S H Zigmond¹, E F Foxman, J E Segall

¹University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Current Protocols in Cell Biology

|January 30, 2008

Summary

This summary is machine-generated.

This study details chemotaxis assays for identifying chemoattractants and analyzing cell responses. Various methods distinguish chemotaxis from chemokinesis and assess cellular behavior.

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Area of Science:

Cell Biology
Biochemistry
Biophysics

Background:

Chemotaxis is a fundamental cellular process involving directed cell movement in response to chemical signals.
This response encompasses chemoattractant detection, signal transduction, and subsequent changes in cell motility and adhesion.
Understanding chemotaxis is crucial for various biological phenomena, including immune response and development.

Purpose of the Study:

To describe a comprehensive suite of chemotaxis assays.
To enable the identification of chemoattractants through individual and large-scale screening.
To differentiate chemotaxis from chemokinesis and analyze cellular behavioral and biochemical responses.

Main Methods:

Utilizing filter, under agarose, and small population assays for monitoring large cell group behavior.
Employing bridge, pipet, and upshift assays for analyzing single-cell responses.
Describing methods to measure chemoattractant concentration and cellular signal transduction.

Main Results:

Demonstration of assays capable of identifying chemoattractants.
Distinction between chemotaxis (directed movement) and chemokinesis (increased random movement).
Analysis of cellular behavioral and biochemical changes induced by chemoattractants.

Conclusions:

The described assays provide versatile tools for studying chemotaxis.
These methods facilitate both high-throughput screening and detailed single-cell analysis.
The assays are valuable for investigating the complex mechanisms underlying cellular responses to chemical stimuli.