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Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

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Related Experiment Video

Updated: Jul 7, 2026

Staining Proteins in Gels
10:55

Staining Proteins in Gels

Published on: July 8, 2008

Staining proteins in gels.

E C Dell'Angelica1, J S Bonifacino

  • 1National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA.

Current Protocols in Cell Biology
|January 30, 2008
PubMed
Summary
This summary is machine-generated.

This study details protein visualization techniques post-gel electrophoresis. Methods include Coomassie blue, silver staining, SYPRO Ruby fluorescence, and reversible zinc staining for clear protein band detection.

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Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
06:24

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Published on: April 21, 2019

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Last Updated: Jul 7, 2026

Staining Proteins in Gels
10:55

Staining Proteins in Gels

Published on: July 8, 2008

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
07:47

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Published on: August 14, 2009

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
06:24

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Published on: April 21, 2019

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Protein visualization is crucial after separation by gel electrophoresis.
  • Various staining methods offer different sensitivities and detection principles.

Purpose of the Study:

  • To present protocols for multiple protein staining techniques.
  • To compare the characteristics of different staining methods for protein visualization.

Main Methods:

  • Coomassie blue staining for visible blue bands.
  • Silver staining for high-sensitivity brown/black bands.
  • SYPRO Ruby staining for fluorescent protein detection.
  • Reversible zinc staining for clear spots against an opaque background.

Main Results:

  • Coomassie blue provides a common, clear background with blue bands.
  • Silver staining offers enhanced sensitivity but is technically demanding.
  • SYPRO Ruby enables fluorescent visualization of protein bands.
  • Zinc staining allows for reversible detection of protein bands.

Conclusions:

  • Multiple staining methods exist for protein visualization after gel electrophoresis.
  • The choice of staining method depends on sensitivity and application requirements.
  • These techniques are applicable to various gel electrophoresis formats, including isoelectric focusing.