Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunoprecipitation.

J S Bonifacino1, E C Dell'Angelica, T A Springer

  • 1National Institute of Child Health and Human Development, Bethesda, Maryland, USA.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

BLOC-1 deficiency causes alterations in amino acid profile and in phospholipid and adenosine metabolism in the postnatal mouse hippocampus.

Scientific reports·2017
Same author

Selective Targeting of High-Affinity LFA-1 Does Not Augment Costimulation Blockade in a Nonhuman Primate Renal Transplantation Model.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2016
Same author

Linker regions and flexibility around the metalloprotease domain account for conformational activation of ADAMTS-13.

Journal of thrombosis and haemostasis : JTH·2015
Same author

Biology and physics of von Willebrand factor concatamers.

Journal of thrombosis and haemostasis : JTH·2011
Same author

Temperature control during laser vessel welding.

Applied optics·2010
Same author

The dysbindin-containing complex (BLOC-1) in brain: developmental regulation, interaction with SNARE proteins and role in neurite outgrowth.

Molecular psychiatry·2009
Same journal

A Comprehensive Experimental Guide to Studying Cross-Presentation in Dendritic Cells In Vitro.

Current protocols in immunology·2020
Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020
Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020
Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020
Same journal

Monoclonal Antibodies to Human Cell Surface Antigens.

Current protocols in immunology·2020
Same journal

Protocols for Experimental Sjögren's Syndrome.

Current protocols in immunology·2020
See all related articles

This study details immunoprecipitation (IP) protocols for isolating specific antigens. Methods cover radiolabeled and unlabeled proteins, including membrane-bound and nonspecifically associated antigens, for subsequent analysis.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Immunoprecipitation (IP) is a crucial technique for isolating specific antigens from complex biological samples.
  • The process involves sequential steps including cell lysis, antibody-antigen binding, complex precipitation, washing, and antigen dissociation.
  • Accurate antigen isolation is vital for downstream analyses like electrophoresis.

Purpose of the Study:

  • To provide detailed protocols for performing immunoprecipitation.
  • To describe methods for isolating both radiolabeled and unlabeled protein antigens.
  • To cover variations for different antigen types, including membrane-bound and nonspecifically associated proteins.

Main Methods:

  • Detailed basic protocol for immunoprecipitation of radiolabeled antigens using antibody-Sepharose conjugates.

Related Experiment Videos

  • Alternate protocols for precipitating soluble immune complexes and isolating antigens.
  • Methods utilizing various affinity reagents such as anti-immunoglobulin serum, protein A/G-Sepharose, and Staphylococcus aureus bacteria.

    • Main Results:

    • Successful immunoprecipitation of radiolabeled antigens using specific antibodies covalently linked to Sepharose.
    • Demonstration of methods for isolating soluble immune complexes.
    • Adaptable protocols for immunoprecipitating unlabeled protein antigens and those associated with other proteins.

    Conclusions:

    • The described protocols offer a comprehensive guide to immunoprecipitation for diverse research applications.
    • These methods enable the efficient isolation and subsequent analysis of specific antigens.
    • The unit provides versatile techniques for researchers in molecular biology and immunology.