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Related Concept Videos

Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Precipitation and Co-precipitation01:17

Precipitation and Co-precipitation

Precipitation and coprecipitation methods can be used to separate a mixture of ions in a solution. In qualitative inorganic analysis, ions that form sparingly soluble precipitates with the same reagent are separated based on the differences in solubility products. For example, consider the separation of Cu(II) and Fe(II) ions by precipitation as insoluble sulfides. First, copper(II) sulfide is precipitated by the addition of acidic H2S, where the dissociation of H2S is suppressed. Adding H2S...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Types of Coprecipitation01:10

Types of Coprecipitation

Coprecipitation is the contamination of a precipitate by otherwise soluble species and occurs via different processes. In colloidal precipitates, coprecipitation occurs via surface adsorption. For instance, barium sulfate has a primary layer of adsorbed barium ions and a secondary layer of nitrate counterions. This results in contamination of the precipitate by barium nitrate.
Sometimes, ions in a crystal lattice can undergo isomorphous replacement by inclusions of similar charge and size. For...

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Related Experiment Video

Updated: Jul 7, 2026

Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions
07:16

Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions

Published on: January 5, 2024

Immunoprecipitation.

J S Bonifacino1, E C Dell'Angelica

  • 1National Institute of Child Health and Human Development, Bethesda, Maryland, USA.

Current Protocols in Cell Biology
|January 30, 2008
PubMed
Summary
This summary is machine-generated.

This study details selective immunoprecipitation (IP) protocols for protein characterization and interaction studies. It includes methods for cell lysis, antibody binding, IP, and enhancing specificity through reprecipitation.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Selective immunoprecipitation (IP) is crucial for protein analysis.
  • Characterizing proteins and their interactions requires reliable IP methods.

Purpose of the Study:

  • To provide clear, step-by-step protocols for performing selective immunoprecipitation.
  • To offer methods for enhancing the specificity of immunoprecipitation.

Main Methods:

  • Detailed protocols for preparing cell and yeast lysates.
  • Methods for antibody immobilization on solid matrices.
  • Procedures for executing the immunoprecipitation reaction.
  • A technique for antigen reprecipitation to increase specificity.

Main Results:

  • Comprehensive protocols enable effective protein characterization.
  • The methods facilitate the study of protein-protein interactions.
  • Reprecipitation significantly enhances the specificity of the immunoprecipitation technique.

Conclusions:

  • The provided protocols offer a robust framework for selective immunoprecipitation.
  • These methods are valuable for researchers studying protein function and interactions.
  • Enhanced specificity through reprecipitation improves data reliability.