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Related Concept Videos

Overview of Fatty Acid Metabolism01:28

Overview of Fatty Acid Metabolism

Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Fats as Energy Storage Molecules01:06

Fats as Energy Storage Molecules

Triglycerides are a form of long-term energy storage molecules. They are made of glycerol and three fatty acids. To obtain energy from fat, triglycerides must first be broken down by hydrolysis into their two principal components, fatty acids and glycerol. This process, called lipolysis, takes place in the cytoplasm. The resulting fatty acids are oxidized by β-oxidation into acetyl-CoA, which is used by the Krebs cycle. The glycerol that is released from triglycerides after lipolysis directly...
Fats as Energy Storage Molecules01:06

Fats as Energy Storage Molecules

Triglycerides are a form of long-term energy storage molecules. They are made of glycerol and three fatty acids. To obtain energy from fat, triglycerides must first be broken down by hydrolysis into their two principal components, fatty acids and glycerol. This process, called lipolysis, takes place in the cytoplasm. The resulting fatty acids are oxidized by β-oxidation into acetyl-CoA, which is used by the Krebs cycle. The glycerol that is released from triglycerides after lipolysis directly...
Lipid Catabolism01:25

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
Metabolic States of the Body: Fasting and Starvation01:24

Metabolic States of the Body: Fasting and Starvation

During the initial hours of fasting, the body uses up its glycogen stores as an energy source. Once these glycogen reserves are depleted, the body begins breaking down stored triglycerides and structural proteins. During this stage, glycerol becomes a key substrate for gluconeogenesis, while free fatty acids undergo beta-oxidation to provide energy for tissues, such as skeletal muscle. In the fasting state, the body spares protein breakdown as much as possible to conserve muscle and structural...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Related Experiment Video

Updated: Jul 7, 2026

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry
07:27

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

Published on: April 9, 2021

Metabolic labeling with fatty acids.

C S Jackson1, A I Magee

  • 1National Institute For Medical Research, London, United Kingdom.

Current Protocols in Cell Biology
|January 30, 2008
PubMed
Summary
This summary is machine-generated.

Radiolabeled fatty acids offer an alternative for protein labeling. This research details methods for fatty acid labeling, linkage analysis, and identification of bound fatty acids in cell extracts.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Protein labeling is crucial for studying protein function and localization.
  • Traditional labeling methods may have limitations in certain applications.
  • Fatty acid acylation is a common post-translational modification involved in protein function.

Purpose of the Study:

  • To provide a comprehensive unit on methods for covalent attachment of radiolabeled fatty acids to proteins.
  • To enable researchers to analyze the linkage, level, and identity of protein-bound fatty acids.
  • To offer an alternative strategy for protein labeling and characterization.

Main Methods:

  • Biosynthetic labeling of proteins using radiolabeled fatty acids (e.g., [3H]myristate, palmitate).
  • Analysis of the covalent linkage between fatty acids and proteins.
  • Quantification of total protein-bound fatty acid levels in cell extracts.
  • Identification of the specific fatty acids bound to proteins.

Main Results:

  • Established protocols for effective biosynthetic fatty acid labeling of proteins.
  • Validated methods for analyzing the nature and extent of fatty acid-protein conjugation.
  • Demonstrated techniques for assessing overall fatty acid incorporation into cellular proteins.
  • Provided a framework for identifying the types of fatty acids attached to proteins.

Conclusions:

  • Covalent attachment of radiolabeled fatty acids is a viable and versatile method for protein labeling.
  • The described methods facilitate detailed analysis of fatty acid acylation in biological systems.
  • This unit serves as a valuable resource for researchers investigating protein lipidation and function.