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Published on: January 26, 2012
Metabolic labeling of prenyl and carboxyl-methyl groups
1National Institute of Medical Research, London, United Kingdom.
Abstract:
This unit provides protocols for prenylation and carboxy-methylation of proteins in cultured cells. These modifications often accompany fatty acid acylation. Cultured cells can be labeled biosynthetically using radiolabeled mevalonate, a precursor, to label intermediates that are incorporated as prenoids--e.g., farnesyl and geranylgeranyl. Carboxy-methylation often accompanies prenylation. The methyl group can be labeled using [(3)H-methyl]methionine.
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