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Related Experiment Video

Updated: Jul 7, 2026

Evaluation of the Storage Stability of Extracellular Vesicles

Evaluation of the Storage Stability of Extracellular Vesicles

Published on: May 22, 2019

Isolation of GLUT4 storage vesicles.

Konstantin V Kandror¹, Paul F Pilch

¹Boston University School of Medicine, Boston, Massachusetts, USA.

Current Protocols in Cell Biology

|January 30, 2008

Summary

This study details a method to isolate glucose transporter type 4 (GLUT4) vesicles from adipocytes and muscle cells. This technique uses antibody-based purification for high-purity vesicle isolation, crucial for understanding glucose transport.

Area of Science:

Cell biology
Biochemistry
Physiology

Background:

Glucose transporter type 4 (GLUT4) is critical for insulin-stimulated glucose uptake in adipocytes and skeletal muscle.
Understanding GLUT4 vesicle trafficking is key to metabolic research and diabetes studies.
Existing methods for isolating GLUT4 vesicles lack sufficient purity and efficiency.

Purpose of the Study:

To describe a robust method for the immunoisolation of GLUT4-containing vesicles.
To enable high-purity isolation of GLUT4 vesicles from major insulin-responsive tissues.
To provide a protocol applicable to various cell types and experimental conditions.

Main Methods:

Cellular homogenization and differential centrifugation to obtain intracellular membranes.

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Isolation of Tissue Extracellular Vesicles from the Liver

Isolation of Tissue Extracellular Vesicles from the Liver

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Isolation of Tissue Extracellular Vesicles from the Liver

Isolation of Tissue Extracellular Vesicles from the Liver

Published on: August 21, 2019

Utilizing immobilized monoclonal antibodies for specific affinity purification of GLUT4 vesicles.

Application of protocols to cultured adipocytes, primary adipocytes, and skeletal muscle tissue.

Main Results:

Successful isolation of GLUT4-containing vesicles with very high purity.
Demonstration of the protocol's applicability across different cell sources.
Establishment of a reliable method for studying GLUT4 vesicle dynamics.

Conclusions:

The described immunoisolation technique provides a powerful tool for investigating GLUT4 vesicle biology.
This method facilitates research into insulin signaling and glucose homeostasis.
The protocol's versatility supports broad applications in metabolic research.