Related Experiment Video
Updated: Jul 7, 2026
07:58
Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
Application of DNA-based methods in typing Vibrio cholerae strains
Durg V Singh1, Harapriya Mohapatra
1Institute of Life Sciences, Nalco Square, Bhubaneswar-751023, India. singhdv@ils.res.in
Future Microbiology
|January 31, 2008
Summary
DNA-based genotyping methods are essential for understanding Vibrio cholerae. These techniques reveal strain subtypes, infection origins, and virulence, aiding in disease control and environmental monitoring.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Molecular biology techniques analyzing microbial DNA are fundamental in biological research.
- Genotyping provides insights into organism subtypes, infection sources, and virulence.
- These methods are crucial for monitoring vaccination programs and understanding pathogen evolution.
Purpose of the Study:
- To review DNA-based techniques for the molecular analysis of Vibrio cholerae.
- To discuss the applications of these techniques in understanding V. cholerae.
- To explore future directions in V. cholerae molecular epidemiology.
Main Methods:
- Pulsed-field gel electrophoresis (PFGE)
- Ribotyping
- CTX typing
- Amplified fragment length polymorphism (AFLP)
- Enterobacterial Intergenic Consensus Sequence-PCR (ERIC-PCR)
- Multilocus Sequence Typing (MLST)
- Microarray methods
Main Results:
- These methods determine genetic changes in toxigenic and nontoxigenic Vibrio cholerae strains.
- They help identify the origin of infections and relationships between clinical and environmental strains.
- Simultaneous detection of CTX prophage copy number/types and persistence genes is possible.
Conclusions:
- DNA-based techniques are vital for comprehensive molecular analysis of V. cholerae.
- Applications include tracking strain origins, virulence, and environmental persistence.
- Continued development of these methods will enhance our understanding and control of cholera.
More Related Videos
Related Concept Videos
01:28Methods of Classification and Identification
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
01:29Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
01:20Applications of Molecular Taxonomy
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
01:24Automated Microbial Diagnostics
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
01:25Cholera
Cholera is an acute gastrointestinal disease caused by the Gram-negative bacterium Vibrio cholerae. It is transmitted primarily via the fecal-oral route through the ingestion of contaminated water or food.Vibrio cholerae is a motile, Gram-negative bacterium of the family Vibrionaceae, primarily associated with waterborne outbreaks in areas with inadequate sanitation. Although over 200 serogroups of V. cholerae exist, only O1 and O139 are responsible for epidemic cholera. The O1 serogroup,...