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Updated: Jul 7, 2026
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High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
Influenza detection and subtyping by reverse transcriptase polymerase chain reaction-restriction fragment length
Emerson Carraro1, Aripuanã Sakurada Aranha Watanabe, Daniel Ferreira Lima Neto
1Clinical Virology Laboratory, Infectious Diseases Unit, Medicine Department, Universidade Federal de São Paulo, São Paulo, SP 04039-032, Brazil.
Diagnostic Microbiology and Infectious Disease
|February 1, 2008
Abstract:
A duplex reverse transcriptase polymerase chain reaction-restriction fragment length polymorphism for influenza virus subtyping was applied to 412 patient samples. The assay was able to discriminate all 47 influenza A H1N1 and H3N2 viruses. This rapid technique assessed if positive samples were current circulating strains or an emergent one and could be used as the 1st test in prepandemic stages.
