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Related Concept Videos

DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
DNA Topoisomerases02:02

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types.  Type I...
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...

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Related Experiment Video

Updated: May 10, 2026

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
11:42

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

DNA-guided crystallization of colloidal nanoparticles.

Dmytro Nykypanchuk1, Mathew M Maye, Daniel van der Lelie

  • 1Center for Functional Nanomaterials, Brookhaven National Laboratory, Upton, New York 11973, USA.

Nature
|February 1, 2008
PubMed
Summary

Researchers created 3D crystalline gold nanoparticle assemblies using DNA interactions. This DNA-mediated crystallization approach enables reversible, temperature-tuneable structures for advanced metamaterials.

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biotechnology

Background:

  • Self-assembly offers precise fabrication of ordered nanostructures.
  • Designing complex, multi-component systems via self-assembly remains a challenge.
  • Existing DNA-based nanoparticle assembly is limited to 1D and 2D, with 3D methods yielding amorphous structures.

Purpose of the Study:

  • To develop a method for creating 3D crystalline nanoparticle assemblies using DNA interactions.
  • To investigate the properties and formation of these 3D assemblies.
  • To advance the creation of novel metamaterials and hybrid systems.

Main Methods:

  • Attaching complementary DNA molecules to gold nanoparticles.
  • Utilizing DNA hybridization to mediate nanoparticle self-assembly in three dimensions.
  • Employing temperature cycling to control reversible crystallization.

Main Results:

  • Successfully formed 3D crystalline assemblies of gold nanoparticles.
  • Demonstrated reversible crystal formation during heating and cooling cycles.
  • Characterized a temperature-tuneable, body-centred-cubic lattice structure with an open volume.

Conclusions:

  • DNA-mediated crystallization is a viable strategy for creating ordered 3D nanoparticle assemblies.
  • This approach facilitates the design of new multicomponent metamaterials.
  • Provides insights into DNA design for hybrid systems with addressable interactions.