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Growth kinetics of Bu3 cells secreting HBsAg in microcarrier culture
1Research Institute of Biochemical Engineering, East China University of Chemical Technology, Shanghai.
Chinese Journal of Biotechnology
|January 1, 1991
Abstract:
The genetically engineered mammalian cell (Bu3) secreting Hepatitis B surface antigen (HBsAg) was cultured on microcarriers semi-continuously. The growth model of Bu3 cells propagating on microcarriers, glucose consumption and lactate accumulation models were established. It is illustrated that the technology of anchorage-dependent Bu3 cell culture on microcarriers will give high yield and high HBsAg expression.