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[Extract process of cyclic adenosinemonophosphate (cAMP) in Ziziphus jujuba]
Ming Li1, Guo-lin Yang, Sha Mi
1Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China.
Objective:
To study the extract process of Cyclic adenosinemonophosphate (cAMP) in Ziziphus jujuba.
Methods:
CAMP was extracted with water, separated and purificated by ion exchange and silica gel G column chromatography.
Results:
Thin layer chromatography (TLC) of the extracts and control cAMP showed the same Rf 0.75; The UV-absorption of the extracts and control cAMP had maximal absorb peak at 260 nm and the least absorb value at 230 nm; Infra-red spectrum of the extracts was indistinguishable from that of control cAMP; Retention time of the extracts by high-performance liquid chromatography (HPLC) determinate was 5.237 minute,unsettled impurity peak,content was 97%. Control cAMP was 5.350 minute,their retention time were of little difference.
Conclusion:
Chemistry structure of this extract with that of control cAMP is the same.
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