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Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
Published on: January 16, 2017
Integration of various stacking processes in carrier ampholyte-based capillary electrophoresis
Jean-Marc Busnel1, Thomas Le Saux, Stéphanie Descroix
1Laboratoire Environnement et Chimie Analytique, UMR CNRS 7121, ESPCI, 10 rue Vauquelin, 75005 Paris, France. jean-marc.busnel@epfl.ch
Journal of Chromatography. A
|February 2, 2008
Summary
Carrier ampholyte-based capillary electrophoresis successfully integrated field-amplified techniques for online analyte preconcentration. This method achieved significant sensitivity enhancement, comparable to traditional conductive buffers, with factors up to several thousands.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Carrier ampholyte-based background electrolytes offer unique properties for capillary electrophoresis.
- Field-amplified techniques are crucial for enhancing analyte detection sensitivity.
Purpose of the Study:
- To integrate field-enhanced sample stacking, field-enhanced sample injection, and electrokinetic supercharging with carrier ampholyte-based capillary electrophoresis.
- To evaluate the efficiency of online preconcentration using these integrated techniques in low-conductivity buffers.
Main Methods:
- Integration of field-enhanced sample stacking, field-enhanced sample injection, and electrokinetic supercharging.
- Capillary electrophoresis using carrier ampholyte-based background electrolytes.
- Analysis of diverse test sample mixtures to assess performance.
Main Results:
- Successful integration of field-amplified preconcentration techniques with carrier ampholyte systems.
- Demonstrated efficient online preconcentration of analytes despite low buffer conductivity.
- Achieved sensitivity enhancement factors ranging from 50 to several thousands, comparable to conductive buffers.
Conclusions:
- Carrier ampholyte-based buffers are effective for online preconcentration in capillary electrophoresis using field-amplified techniques.
- These methods provide substantial sensitivity enhancements, broadening their applicability in trace analysis.
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