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Related Concept Videos

Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Mismatch Repair01:36

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Mismatch Repair01:36

Mismatch Repair

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Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...

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Related Experiment Video

Updated: Jul 7, 2026

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
11:40

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Published on: June 25, 2013

Functional interactions between Sae2 and the Mre11 complex.

Hee-Sook Kim1, Sangeetha Vijayakumar, Mike Reger

  • 1Laboratory of Chromosome Biology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.

Genetics
|February 5, 2008
PubMed
Summary
This summary is machine-generated.

Sae2 protein antagonizes the Mre11 complex

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Last Updated: Jul 7, 2026

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
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Published on: June 25, 2013

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Assessment of DNA Double Strand Break Repair Activity Using High-throughput and Quantitative Luminescence-Based Reporter Assays

Published on: June 14, 2024

Area of Science:

  • Molecular Biology
  • DNA repair
  • Cellular signaling

Background:

  • The Mre11 complex is crucial for DNA double-strand break (DSB) repair, meiotic recombination, and DNA damage checkpoint pathways.
  • Sae2 protein has dual roles, potentially impairing Mre11 nuclease function while activating Mre11-dependent DNA damage signaling.

Purpose of the Study:

  • To investigate the regulatory mechanism of Sae2 on the Mre11 complex.
  • To understand how Sae2 influences both DNA repair and checkpoint functions.

Main Methods:

  • Screening for Sae2 alleles with specific checkpoint and nuclease functions.
  • Phenotypic characterization of Sae2 alleles.
  • Investigating Sae2 oligomerization and its dependence on DNA damage.

Main Results:

  • Sae2 overexpression blocks the Tel1-Mre11 (TM) pathway, indicating Sae2 antagonizes Mre11 checkpoint functions.
  • Identified Sae2 alleles that separate checkpoint and nuclease functions.
  • Sae2 functions as a multimer, influencing Mre11 nuclease specificity.
  • Sae2 oligomerization occurs independently of DNA damage and is essential for regulating Mre11 functions.

Conclusions:

  • Sae2 acts as a multimer to regulate Mre11 complex functions, including nuclease activity and checkpoint signaling.
  • Sae2's oligomerization is critical for its dual role in DNA repair and damage response.