Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

11.0K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.0K
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

1.2K
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
1.2K
Internal Energy02:00

Internal Energy

36.5K
The total of all possible kinds of energy present in a substance is called the internal energy (U), sometimes symbolized as E. Suppose a system with initial internal energy, Uinitial, undergoes a change in energy (transfer of work or heat), and the final internal energy of the system is Ufinal. Change in internal energy equals the difference between Ufinal and Uinitial.
36.5K
Internal Energy01:29

Internal Energy

6.8K
The internal energy of a thermodynamic system is the sum of the kinetic and potential energies of all the molecules or entities in the system. The kinetic energy of an individual molecule includes contributions due to its rotation and vibration, as well as its translational energy. The potential energy is associated only with the interactions between one molecule and the other molecules of the system. Neither the system's location nor its motion is of any consequence as far as the internal...
6.8K
Internal Receptors01:31

Internal Receptors

74.3K
Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
74.3K
Reflection of Waves01:07

Reflection of Waves

4.5K
When a wave travels from one medium to another, it gets reflected at the boundary of the second medium. A common example of this is when a person yells at a distance from a cliff and hears the echo of their voice. The sound waves (longitudinal waves) traveling in the air are reflected from the bounding cliff. Similarly, flipping one end of a string whose other end is tied to a wall causes a pulse (transverse wave) to travel through the string, which gets reflected upon reaching the wall. In...
4.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cardiomyocyte-derived USP20 mitigates myocardial ischemia/reperfusion injury through deubiquitinating GRP78.

Theranostics·2026
Same author

Molecular basis for the dopamine reuptake and inhibition mechanism of human dopamine transporter.

Structure (London, England : 1993)·2026
Same author

Cohort profile: PeRiOperative sTress risk assEssment and Clinical decision cohorT (PROTECT), a multi-center observational study based on real-world data.

BMC geriatrics·2026
Same author

SIRT5 mitigates metabolic abnormality in murine models of metabolic dysfunction-associated steatotic liver disease.

Life medicine·2026
Same author

Resonant light manipulation with the GaAs nanopillar array for green band applications.

Applied optics·2026
Same author

Programmable viscoelastic hydrogels exhibit antimicrobial and regenerative properties to promote cell migration, wound healing, and tissue remodeling.

Microsystems & nanoengineering·2026
Same journal

Multi-module collaborative optimization-driven fast speckle correlation imaging in variable environments.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Secrecy performance analysis of NOMA-UWOC systems over a vertically stratified WGG oceanic turbulence channel.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Backscattering of plane waves in a composite system containing a rough surface and anisotropic scatterers.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Aspherical surface construction methods based on extended Jacobi polynomials.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

OCT sidelobe suppression method based on dual-path phase sinusoidal modulation and minimum value fusion.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Optical design concepts using wavelength-selective diffractive optics to enable miniaturized multimodal endoscopic imaging across separated spectral ranges.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
See all related articles

Related Experiment Video

Updated: Jan 20, 2026

Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence TIRF Microscopy
08:44

Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence TIRF Microscopy

Published on: July 20, 2022

4.0K

MUSIC imaging applied to total internal reflection tomography.

Xudong Chen1

  • 1Department of Electrical and Computer Engineering, National University of Singapore, Singapore. elechenx@nus.edu.sg

Journal of the Optical Society of America. A, Optics, Image Science, and Vision
|February 5, 2008
PubMed
Summary
This summary is machine-generated.

This study uses the MUSIC imaging method for total internal reflection tomography to locate small, anisotropic scatterers. A fast analytical approach efficiently solves the nonlinear inverse scattering problem, even with multiple scattering effects.

More Related Videos

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
06:43

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

4.0K
Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy
10:19

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Published on: January 21, 2019

6.9K

Related Experiment Videos

Last Updated: Jan 20, 2026

Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence TIRF Microscopy
08:44

Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence TIRF Microscopy

Published on: July 20, 2022

4.0K
Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
06:43

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

4.0K
Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy
10:19

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Published on: January 21, 2019

6.9K

Area of Science:

  • Electromagnetic theory
  • Inverse scattering problems
  • Tomographic imaging

Background:

  • Total internal reflection (TIR) tomography is a technique used for imaging.
  • Locating small anisotropic spherical scatterers presents challenges due to multiple scattering and nonlinear inverse problems.

Purpose of the Study:

  • To apply the Multiple Signal Classification (MUSIC) imaging method within TIR tomography.
  • To develop a fast analytical approach for solving the nonlinear inverse scattering problem.
  • To analyze factors affecting imaging resolution, including wave types, polarization, and noise.

Main Methods:

  • Application of the MUSIC algorithm for inverse scattering.
  • Development of a fast analytical method to solve the nonlinear inverse problem without iterative forward modeling.
  • Consideration of multiple scattering effects between scatterers.

Main Results:

  • Successfully determined the locations of small anisotropic spherical scatterers using MUSIC imaging.
  • The proposed analytical approach efficiently solved the nonlinear inverse scattering problem.
  • Investigated the impact of propagating and evanescent waves, wave polarization, substrate separation, and noise on imaging resolution.

Conclusions:

  • The MUSIC method is effective for locating scatterers in TIR tomography.
  • A fast analytical approach can efficiently solve complex nonlinear inverse scattering problems.
  • Understanding wave properties and noise is crucial for optimizing imaging resolution.