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Lipid-Protein Membrane Structure-Function Characterization using Droplet Interface Bilayers
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Pressure induces interdigitation differently in DPPC and DPPG.

Harpreet Singh1, Jason Emberley, Michael R Morrow

  • 1Department of Physics and Physical Oceanography, Memorial University of Newfoundland, A1B 3X7, St. John's, NF, Canada.

European Biophysics Journal : EBJ
|February 6, 2008
PubMed
Summary

The study compared dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG) bilayer phase behavior under high pressure. DPPG bilayers interdigitate at lower pressures than DPPC bilayers, highlighting the influence of headgroup interactions.

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Published on: October 15, 2015

Area of Science:

  • Biophysics
  • Materials Science

Background:

  • Understanding lipid bilayer phase behavior is crucial for membrane biophysics and drug delivery.
  • Phospholipids like DPPC and DPPG are key components of biological membranes.

Purpose of the Study:

  • To compare the pressure-temperature phase behavior of chain-perdeuterated dipalmitoylphosphatidylcholine (DPPC-d62) and dipalmitoylphosphatidylglycerol (DPPG-d62) bilayers.
  • To investigate the influence of headgroup charge on lipid bilayer phase transitions under pressure.

Main Methods:

  • 2H nuclear magnetic resonance (NMR) spectroscopy.
  • Quadrupole echo decay measurements.
  • Variable temperature and high-pressure experiments (ambient to 196 MPa, -25 to 60°C).

Main Results:

  • At ambient pressure, DPPC-d62 and DPPG-d62 showed nearly identical phase behavior.
  • At 196 MPa, both lipids exhibited a transition from an interdigitated gel phase to a non-interdigitated gel phase, and then to a chain-immobilized ordered phase with decreasing temperature.
  • DPPG-d62 showed evidence of interdigitation at 85 MPa, while DPPC-d62 did not, indicating a lower pressure threshold for interdigitation in DPPG.

Conclusions:

  • DPPG bilayers exhibit interdigitation at significantly lower pressures (starting at 60 MPa) compared to DPPC bilayers (minimum ~150 MPa).
  • This difference underscores the critical role of headgroup interactions in modulating lipid bilayer phase behavior under pressure.
  • Bilayer phase diagrams reveal distinct pressure-dependent transitions influenced by lipid headgroup chemistry.