Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Polytene Chromosomes

Polytene Chromosomes

Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also regularly...

Polytene Chromosomes

Polytene Chromosomes

Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also regularly...

Structure of a Gene

Structure of a Gene

A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...

Cis-regulatory Sequences

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

Cis-regulatory Sequences

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Functional determinism amid taxonomic stochasticity: insights into rules governing the assembly of algal-microbial symbioses.

Applied and environmental microbiology·2026

Same author

[Recommendations for the Standardization of Forensic Microbiological Analysis].

Fa yi xue za zhi·2026

Same author

Current mechanisms and techniques for placement of self-expandable metal stents in acute colonic obstruction.

World journal of gastrointestinal surgery·2025

Same author

Quercetin Confers Protection against Sepsis-Related Acute Respiratory Distress Syndrome by Suppressing ROS/p38 MAPK Pathway.

Chinese journal of integrative medicine·2025

Same author

Seventy Years of Passing on the Torch, Striving Forward in the New Era - Thoughts on the Construction of Forensic Medicine at Sun Yat-sen University.

Fa yi xue za zhi·2025

Same author

Photocatalytic three-component reaction for synthesis of dithio-carbamates.

Organic & biomolecular chemistry·2025

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

Comparing the Frequency Effect Between the Lexical Decision and Naming Tasks in Chinese

Comparing the Frequency Effect Between the Lexical Decision and Naming Tasks in Chinese

Published on: April 1, 2016

Development of a five ChX STRs loci typing system.

Qiu-ling Liu¹, De-jian Lv, Xiang-lin Wu

¹Faculty of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, No.74 ZhongShan Road II, Guangzhou 510089, China. liuql@mail.sysu.edu.cn

International Journal of Legal Medicine

|February 6, 2008

Summary

This study evaluated five X-chromosomal short tandem repeat (ChrX STR) loci for forensic use. The developed multiplex PCR system offers high polymorphism, proving valuable for identification and paternity testing, especially in challenging cases.

More Related Videos

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Published on: May 12, 2023

Related Experiment Videos

Last Updated: Jul 7, 2026

Comparing the Frequency Effect Between the Lexical Decision and Naming Tasks in Chinese

Comparing the Frequency Effect Between the Lexical Decision and Naming Tasks in Chinese

Published on: April 1, 2016

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Published on: May 12, 2023

Area of Science:

Forensic Genetics
Human Genetics
Molecular Biology

Background:

Short tandem repeat (STR) markers are crucial for forensic identification.
X-chromosomal STRs (ChrX STRs) are particularly useful for relationship testing, including paternity analysis.
Assessing the forensic value of specific ChrX STR loci is essential for improving identification capabilities.

Purpose of the Study:

To investigate the genetic polymorphism of five specific ChrX STR loci (DXS7132, DXS981, DXS6803, DXS6809, and DXS6789).
To evaluate the utility of these loci in a multiplex polymerase chain reaction (PCR) system for forensic applications.
To determine the effectiveness of this ChrX STR multiplex system for forensic identification and paternity testing in a Chinese Han population.

Main Methods:

Development of a fluorescent multiplex PCR assay for simultaneous amplification of five ChrX STR loci.
Genotyping of 827 unrelated individuals from the Han nationality in China.
Statistical analysis of polymorphism information content for forensic evaluation.

Main Results:

The five ChrX STR loci exhibited high levels of polymorphism within the studied population.
The multiplex PCR system successfully amplified all five loci, demonstrating its efficiency.
The combined data from these loci provide significant discriminatory power for forensic identification.

Conclusions:

The evaluated ChrX STR loci are highly polymorphic and suitable for forensic applications.
The developed multiplex PCR system is effective for forensic identification and paternity testing.
This system is particularly advantageous for resolving complex paternity cases with limited or no direct paternal evidence.