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[Association of dendritic cell distribution with intima hyperplasia in rats with diabetes mellitus]
Qiang Fu1, Zhi-liang Li, Xiao-hua Fu
1Department of Cardiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. fq-tong@sohu.com
Objective:
To investigate the association of dendritic cell distribution in the peripheral blood, spleen and arterial wall with intimal hyperplasia in rats with diabetes mellitus.
Methods:
Diabetes mellitus was induced in rats by intraperitoneal injection of streptozotocin and high-fat feeding for 8 weeks. Peripheral blood, arterial wall and the spleen were collected from the rats to prepare cell suspensions, in which the proportions of dendritic cells and T cells were determined by flow cytometry.
Results:
The tunica intimal hyperplasia was more obvious in diabetic rats with or without high-fat feeding as compared with that of the control rats (P<0.05), and their dendritic cells decreased significantly in the peripheral blood (P<0.05) but increased in the arterial wall. The percentage of T cells was also increased in the peripheral blood and arterial wall of the diabetic rats.
Conclusion:
Changes in the distribution of dendritic cells and T cells are closely associated with intimal hyperplasia in diabetic rats, suggesting the involvement of dendritic cells and T cells in the formation of atherosclerosis.
