Related Experiment Video
Updated: Jul 7, 2026

Scalable Transfection of Maize Mesophyll Protoplasts
Published on: June 23, 2023
The art and design of genetic screens: maize
1Plant Gene Expression Center, 800 Buchanan Street, Albany, California 94710, USA. hcandela@nature.berkeley.edu
Abstract:
Maize (Zea mays) is an excellent model for basic research. Genetic screens have informed our understanding of developmental processes, meiosis, epigenetics and biochemical pathways--not only in maize but also in other cereal crops. We discuss the forward and reverse genetic screens that are possible in this organism, and emphasize the available tools. Screens exploit the well-studied behaviour of transposon systems, and the distinctive chromosomes allow an integration of cytogenetics into mutagenesis screens and analyses. The imminent completion of the maize genome sequence provides the essential resource to move seamlessly from gene to phenotype and back.

