Related Experiment Video

Updated: Jul 7, 2026

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
07:00

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

Published on: February 26, 2010

Measurement of group delay dispersion of high numerical aperture objective lenses using two-photon excited

J B Guild, C Xu, W W Webb

    Applied Optics
    |January 1, 1997
    PubMed

    More Related Videos

    High Precision FRET at Single-molecule Level for Biomolecule Structure Determination11:24

    High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

    Published on: May 13, 2017

    Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels11:34

    Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

    Published on: September 8, 2016

    Related Concept Videos

    Fluorescence and Phosphorescence: Instrumentation01:25

    Fluorescence and Phosphorescence: Instrumentation

    Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
    Super-resolution Fluorescence Microscopy01:37

    Super-resolution Fluorescence Microscopy

    Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
    Confocal Fluorescence Microscopy01:16

    Confocal Fluorescence Microscopy

    Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...