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Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Related Experiment Video

Updated: Jul 7, 2026

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Fluorescence-based test of fiber-optic continuity.

D P Norwood, C Vinches, J F Anderson

    Applied Optics
    |April 20, 1997
    PubMed
    Summary

    A new method uses fiber optic fluorescence to test pyrotechnic initiation continuity. This ensures reliable fiber function before firing, preventing accidental initiations.

    Area of Science:

    • Optoelectronics
    • Materials Science
    • Chemical Engineering

    Background:

    • Lasers and optical fibers are increasingly used for pyrotechnic initiation.
    • A critical need exists for reliable continuity testing of initiation fibers before pyrotechnic activation.

    Purpose of the Study:

    • To demonstrate the feasibility of an unambiguous optical fiber continuity test for pyrotechnics.
    • To develop a method that ensures the integrity of the initiation pathway prior to firing.

    Main Methods:

    • Utilizing laser-induced fluorescence from a material within or near the pyrotechnic.
    • Employing optical fibers to transmit excitation light and collect the returned fluorescence signal.
    • Analyzing the fluorescence signal for unambiguous confirmation of fiber continuity.

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    Last Updated: Jul 7, 2026

    High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
    13:49

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    Published on: January 11, 2011

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
    10:21

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

    Published on: May 5, 2016

    Design and Fabrication of an Optical Fiber Made of Water
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    Main Results:

    • Proof of concept for a fluorescence-based continuity test was successfully established.
    • The method provides an unambiguous signal, distinguishing between a continuous and a broken fiber.
    • The test is compatible with pyrotechnic initiation systems using optical fibers.

    Conclusions:

    • A novel and unambiguous method for testing optical fiber continuity in pyrotechnic initiation has been developed.
    • This fluorescence-based technique enhances safety and reliability in laser-initiated pyrotechnic systems.
    • The developed method offers a practical solution for pre-initiation verification in demanding applications.