Enhanced initial cell responses to chemically modified anodized titanium
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Updated: Jul 7, 2026
Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants
Published on: August 5, 2021
Ryo Jimbo1, Takashi Sawase, Koumei Baba
1Division of Applied Prosthodontics, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto, Nagasaki, Japan.
Background:
Previously, we reported that anodized porous titanium implants have photocatalytic hydrophilicity. However, this effect was not always sufficient for the significant improvement of bone apposition.
Purpose:
The purpose of this study was to improve the photocatalytic properties of porous titanium implants by the fluoride modification of the anodized titanium dioxide (TiO(2)), and to investigate the initial cell response to it.
Materials And Methods:
The ideal concentration of ammonium hydrogen fluoride (NH(4)F-HF(2)) used in this study was determined by a static water contact angle assay. The ideal concentration of NH(4)F-HF(2) was 0.175%, and experimental disks were treated with this concentration. A pluripotent mesenchymal cell line, C2C12, was cultured on the disks in order to investigate cell attachment, morphology, and proliferation.
Results:
Cell attachment after 30 minutes of culturing was significantly higher for the ultraviolet-irradiated, fluoride-modified anodized TiO(2) (p < .05), and the simultaneous scanning electron microscope observation showed a rather flattened and extended cell morphology. The proliferation rate after 24 hours was also significantly higher for the fluoride-modified anodized TiO(2).
Conclusion:
Fluoride chemical modification enhances the hydrophilic property of the anodized TiO(2) and improves the initial cell response to it.
