Related Experiment Video

Updated: Jul 7, 2026

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
09:01

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

Published on: December 10, 2013

Recombinant VP4 of human rhinovirus induces permeability in model membranes

Matthew P Davis1, Graham Bottley, Lucy P Beales

  • 1Institute for Molecular and Cellular Biology, Faculty of Biological Sciences, The University of Leeds, Leeds LS2 9JT, United Kingdom.

Journal of Virology
|February 8, 2008
PubMed

Insights

Picornavirus entry into cells is unclear. Researchers found that the VP4 protein from human rhinovirus 16 can disrupt liposome membranes, suggesting VP4 is key to viral cell entry.

Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • Picornaviruses are nonenveloped viruses.
  • The mechanism of picornavirus membrane penetration during cell entry is not well understood.
  • The capsid protein VP4 has been implicated in picornavirus cell entry.

Purpose of the Study:

  • To investigate the role of picornavirus VP4 protein in membrane penetration.
  • To provide evidence for VP4's function in viral cell entry.

Main Methods:

  • Produced recombinant VP4 protein from human rhinovirus 16.
  • Assayed the ability of VP4 to associate with and induce permeability in liposomes.

Main Results:

  • Recombinant VP4 associated with liposomes.
  • VP4 induced membrane permeability in intact liposomes.

Conclusions:

  • VP4 plays a significant role in picornavirus membrane penetration.
  • These findings support the hypothesis that VP4 is essential for picornavirus cell entry.

More Related Videos

Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems13:47

Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems

Published on: April 23, 2012

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses11:48

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Published on: April 4, 2019

Related Concept Videos

Inhibitors Of Virion Release01:25

Inhibitors Of Virion Release

Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...
Respiratory Syncytial Virus Disease01:29

Respiratory Syncytial Virus Disease

Human respiratory syncytial virus (RSV) is a widespread pathogen that primarily targets infants and young children but also poses a serious health risk to elderly and immunocompromised individuals. Belonging to the Pneumoviridae family, RSV is a negative-sense, single-stranded RNA virus within the Pneumovirus genus. Its global health burden is significant, with millions of cases annually resulting in hospitalizations and mortality, particularly in resource-limited settings. Although most...