Related Experiment Video
Updated: Jul 7, 2026
05:48
Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
Published on: March 16, 2022
Generation of a conditional null allele for Dmp1 in mouse
Jian Q Feng1, Greg Scott, Dayong Guo
1Department of Biomedical Sciences, Baylor College of Dentistry, Texas A&M Health Science Center, Dallas, Texas 75246, USA. jfeng@bcd.tamhsc.edu
Genesis (New York, N.Y. : 2000)
|February 8, 2008
Summary
Dentin matrix protein1 (DMP1) plays a crucial role in teeth and skeleton development. This study introduces a new mouse model to investigate DMP1
Area of Science:
- Biochemistry
- Genetics
- Developmental Biology
Background:
- Dentin matrix protein1 (DMP1) is vital for dental and skeletal health.
- DMP1 mutations cause hypophosphatemic rickets in humans.
- DMP1's role in various tissues and conditions requires further investigation.
Purpose of the Study:
- To generate a conditional knockout mouse model for DMP1.
- To investigate the function of DMP1 in different tissues and developmental stages.
Main Methods:
- Generation of Dmp1 floxed mice with flanking loxP sites.
- Cre-mediated recombination using Sox2-Cre for early gene deletion.
- Phenotypic analysis of homozygous Cre-recombined null mice.
Main Results:
- Sox2-Cre mediated recombination led to early deletion of DMP1.
- Homozygous Cre-recombined null mice exhibited phenotypes identical to conventional null mice.
- The generated animal model effectively recapitulates DMP1 deficiency.
Conclusions:
- The novel Dmp1 floxed mouse model allows for tissue-specific and temporal investigation of DMP1.
- This model will aid in understanding DMP1's distinct roles in various physiological and pathological contexts.
- Further studies using this model will elucidate DMP1 functions in different tissues and ages.
