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[Extraction and content determination of polysaccharides in Viscum coloratum]
1College of Biology and Environmental Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, China. wangjunjs@tom.com
Objective:
To establish a method for extraction and content determination of polysaccharide in Viscum coloratum.
Method:
Polysaccharide was extracted by hot water, separated by ultrafiltration and ion-exchange chromatography. The content determination was performed at wavelength 490 nm with phenol-sulfuric acid as a chtomo-genic agent.
Result:
The content of polyaccharide in V. coloratum, CVPS-III, and CVPS-III-C were respectively 4.93% (RSD 1.04%, n = 3), 43.28% (RSD 1.39%, n =3), 69.55% (RSD 1.62%, n = 3), and the average recovery was 96.07% (RSD 2.54%, n = 5).
Conclusion:
The method was simple, rapid, and accurate.
