Related Experiment Video
Updated: Jul 7, 2026

07:20
Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR
Published on: February 4, 2018
Bartonella DNA in dog saliva
Ashlee W Duncan1, Ricardo G Maggi, Edward B Breitschwerdt
1North Carolina State University College of Veterinary Medicine, Raleigh, North Carolina, USA.
Emerging Infectious Diseases
|February 9, 2008
Summary
Bartonella bacteria, a growing concern in human and animal health, were detected in dogs. Four species, including Bartonella henselae, were identified using PCR and DNA sequencing from oral swabs.
Area of Science:
- Veterinary Medicine
- Infectious Diseases
- Microbiology
Background:
- Bartonella species are increasingly recognized as pathogens affecting both humans and animals.
- Transmission occurs through arthropod vectors or direct contact with infected animals.
Purpose of the Study:
- To investigate the prevalence of Bartonella species in dogs.
- To identify specific Bartonella species present in canine oral microbiota.
Main Methods:
- Oral swabs were collected from dogs.
- Polymerase Chain Reaction (PCR) and DNA sequencing were employed for pathogen detection and identification.
Main Results:
- Four distinct Bartonella species were identified in the tested canine oral swabs.
- The detected species included Bartonella bovis, Bartonella henselae, Bartonella quintana, and Bartonella vinsonii subspecies berkhoffii.
Conclusions:
- Dogs can harbor multiple Bartonella species in their oral cavities.
- These findings highlight the potential role of dogs as reservoirs for Bartonella infections relevant to public and animal health.
Related Concept Videos
Bacterial Phylum Spirochaetes
Spirochetes, unique bacteria in the phylum Spirochaetes, are gram-negative, motile, tightly coiled, slender, and flexible. They inhabit aquatic sediments and animals, with some causing diseases like syphilis. Spirochetes are classified into eight genera based on habitat, pathogenicity, phylogeny, and characteristics.Their distinctive motility arises from endoflagella, located within the cell’s periplasm. These endoflagella anchor at the cell poles and extend along the cell length, encased by a...
Rabies
Rabies is a lethal zoonotic disease caused by a single-stranded, negative-sense RNA virus of the Lyssavirus genus, within the family Rhabdoviridae. Its primary mode of transmission to humans is through bites or saliva-contaminated scratches from infected mammals such as dogs, bats, raccoons, or foxes. Transmission can also occur if infectious saliva contacts abraded skin or intact mucous membranes, including the conjunctiva.Viral Entry and Early ReplicationOnce introduced at the bite or scratch...
DNA Isolation
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Genomic DNA in Prokaryotes
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...

