Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evaluating the impact of reusable gowns on postoperative infection rates in hand surgery.

Surgery in practice and science·2026

Same author

Photoactive imaging and therapy for colorectal cancer using a CEA-Affimer conjugated Foslip nanoparticle.

Nanoscale·2024

Same author

Network topology mapping of chemical compounds space.

Scientific reports·2024

Same author

Adolescent Contact, Lasting Impact? Lessons Learned From Two Longitudinal Studies Spanning 20 Years of Developmental Science Research With Justice-System-Involved Youths.

Psychological science in the public interest : a journal of the American Psychological Society·2024

Same author

Synthetic Receptors for Early Detection and Treatment of Cancer.

Biosensors·2023

Same author

Hybrid Nanoparticle/DNAzyme Electrochemical Biosensor for the Detection of Divalent Heavy Metal Ions and Cr<sup>3</sup>.

Sensors (Basel, Switzerland)·2023

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

Label-less immunosensor assay for myelin basic protein based upon an ac impedance protocol.

Georgios Tsekenis¹, Goulielmos-Zois Garifallou, Frank Davis

¹Cranfield Health, Cranfield University, Silsoe, Beds, MK45 4DT, UK.

Analytical Chemistry

|February 12, 2008

Summary

This summary is machine-generated.

This study presents a label-less immunosensor for detecting myelin basic protein (MBP). The sensor uses ac impedance to measure MBP concentration, showing a logarithmic relationship for accurate quantification.

More Related Videos

Human Serum Anti-aquaporin-4 Immunoglobulin G Detection by Cell-based Assay

Human Serum Anti-aquaporin-4 Immunoglobulin G Detection by Cell-based Assay

Published on: April 5, 2019

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Related Experiment Videos

Last Updated: Jul 7, 2026

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

Human Serum Anti-aquaporin-4 Immunoglobulin G Detection by Cell-based Assay

Human Serum Anti-aquaporin-4 Immunoglobulin G Detection by Cell-based Assay

Published on: April 5, 2019

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Area of Science:

Biomedical Engineering
Biosensor Technology
Neuroscience

Background:

Myelin basic protein (MBP) is a key biomarker in neurological disorders.
Accurate and sensitive detection of MBP is crucial for diagnosis and monitoring.
Existing detection methods may require labels or complex procedures.

Purpose of the Study:

To develop and characterize a novel label-less immunosensor for MBP detection.
To utilize ac impedance spectroscopy for quantitative analysis of MBP.
To establish a reliable and sensitive method for MBP quantification in buffer solutions.

Main Methods:

Fabrication of screen-printed carbon electrodes modified with polyaniline.
Immobilization of anti-MBP antibodies via avidin-biotin linkage.
Interrogation of the immunosensor using ac impedance spectroscopy.
Analysis of impedance response with varying MBP concentrations.

Main Results:

The real component of impedance increased with increasing MBP concentration.
A logarithmic relationship was observed between MBP concentration and impedimetric response.
Control experiments with non-specific IgG antibodies confirmed specificity and accounted for non-specific adsorption.

Conclusions:

A label-less immunosensor for MBP detection using ac impedance has been successfully developed.
The sensor demonstrates sensitivity and specificity for MBP quantification.
This approach offers a promising method for the label-less detection of biomarkers.