Related Experiment Video
Updated: Jul 7, 2026

Exploring the Pharmacological Action and Molecular Mechanism of Salidroside in Inhibiting MCF-7 Cell Proliferation and Migration
Published on: June 9, 2023
Casein Kinase I epsilon positively regulates the Akt pathway in breast cancer cell lines
1Department of Developmental and Cell Biology, University of California Irvine, Mc Gaugh Hall Room 4340, Irvine, CA 92697, USA.
Abstract:
The Akt pathway is very important in both development and cancer. Here we show that, expression of Casein Kinase I epsilon (CKIepsilon) causes up-regulation of the Akt pathway despite normal protein expression of the pathway inhibitor phosphate and tensin homologue deleted on chromosome ten (PTEN). Conversely, we show that a CKIepsilon/delta-specific inhibitor can inhibit Akt phosphorylation at both Thr308 and Ser473 and drastically reduce phosphorylation of the Akt target Glycogen Synthase Kinase 3beta (GSK3beta). These conclusions were confirmed between MCF7 cells transiently transfected with CKIepsilon and Hs578T cells which already express endogenous CKIepsilon. The results suggest that CKIepsilon is a new positive regulator of the Akt pathway. Here we propose that, rather than inhibiting PTEN function, CKIepsilon positively regulates Akt possibly by inhibiting Protein Phosphatase 2A (PP2A).
Related Concept Videos
PI3K/mTOR/AKT Signaling Pathway
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Negative Regulator Molecules
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Inhibition of Cdk Activity
Positive Regulator Molecules