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Updated: Jul 7, 2026

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Rare Event Detection Using Error-corrected DNA and RNA Sequencing
Published on: August 3, 2018
Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex
Micah Hamady1, Jeffrey J Walker, J Kirk Harris
1Department of Computer Science, UCB 430, University of Colorado, Boulder, Colorado 80309, USA.
Nature Methods
|February 12, 2008
Abstract:
We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.

