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DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
Lytic Cycle of Bacteriophages01:30

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Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the lytic replication...
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Viruses are unique biological entities that blur the boundary between living and non-living systems. Although they lack cellular structure and metabolic processes, they can exhibit characteristics of life when infecting a host. Their defining feature is a nucleic acid core, composed of either DNA or RNA, encapsulated within a protein coat called a capsid. This simple structure allows them to invade host cells and use their machinery for replication efficiently.Viral Structure and...
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Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
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Published on: June 11, 2015

Introduction to vectors derived from filamentous phages.

D Greenstein1, R Brent

  • 1Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.

Current Protocols in Molecular Biology
|February 12, 2008
PubMed
Summary
This summary is machine-generated.

Filamentous phage vectors, derived from filamentous phages, allow recovery of inserted DNA as double-stranded or single-stranded circles. This overview details their lifecycle, development, and applications in molecular biology.

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Last Updated: Jul 7, 2026

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
09:40

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

Following Cell-fate in E. coli After Infection by Phage Lambda
06:10

Following Cell-fate in E. coli After Infection by Phage Lambda

Published on: October 14, 2011

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
08:31

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Published on: May 26, 2013

Area of Science:

  • Molecular Biology
  • Virology
  • Biotechnology

Background:

  • Filamentous phages are widely utilized as vectors in molecular biology.
  • These phage vectors facilitate the recovery of inserted DNA in both double-stranded and single-stranded circular forms.

Purpose of the Study:

  • To provide a comprehensive overview of the filamentous phage lifecycle.
  • To describe the development and application of filamentous phage vectors.

Main Methods:

  • Review of existing literature on filamentous phage biology.
  • Analysis of the mechanisms underlying DNA recovery in double-stranded and single-stranded forms.

Main Results:

  • Filamentous phages possess a unique lifecycle amenable to vector development.
  • The dual recovery forms (dsDNA and ssDNA circles) offer versatility in molecular cloning and manipulation.

Conclusions:

  • Filamentous phage vectors represent a valuable tool in genetic engineering.
  • Understanding their lifecycle is crucial for optimizing their use in various biotechnological applications.