Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Networks

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

Protein-protein Interfaces

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Acquisition of estrogen independence induces TOB1-related mechanisms supporting breast cancer cell proliferation.

Oncogene·2015

Same author

DUSP6 regulates drug sensitivity by modulating DNA damage response.

British journal of cancer·2013

Same author

Aurora kinase A mediates epithelial ovarian cancer cell migration and adhesion.

Oncogene·2013

Same author

A requirement for Nedd9 in luminal progenitor cells prior to mammary tumorigenesis in MMTV-HER2/ErbB2 mice.

Oncogene·2013

Same author

Relationship of increased aurora kinase A gene copy number, prognosis and response to chemotherapy in patients with metastatic colorectal cancer.

British journal of cancer·2012

Same author

Dual inhibition of SRC and Aurora kinases induces postmitotic attachment defects and cell death.

Oncogene·2011

Search research articles

Related Experiment Video

Updated: Jun 30, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Interaction trap/two-hybrid system to identify interacting proteins.

E A Golemis¹, I Serebriiskii, J Gyuris

¹Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA.

Current Protocols in Neuroscience

|September 20, 2008

Summary

This study updates the yeast two-hybrid system with new interaction trap components and compatible libraries. It introduces a novel interaction mating protocol, a valuable technique for researchers using yeast two-hybrid screening.

More Related Videos

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

Published on: February 1, 2010

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Published on: November 17, 2019

Related Experiment Videos

Last Updated: Jun 30, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

Published on: February 1, 2010

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Published on: November 17, 2019

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

The yeast two-hybrid system is a powerful tool for studying protein-protein interactions.
Continuous refinement of experimental systems is crucial for advancing biological research.
Existing protocols may require updates to incorporate new methodologies and reagents.

Purpose of the Study:

To present an updated version of the yeast two-hybrid system.
To provide expanded details on interaction trap components and compatible libraries.
To introduce and detail a new protocol for performing interaction hunts using mating.

Main Methods:

Systematically updated interaction trap component tables.
Incorporated new libraries compatible with the interaction trap system.
Developed and described a novel protocol for interaction mating.

Main Results:

Expanded tables detailing interaction trap components are now available.
Additional compatible libraries have been integrated into the system.
A new, detailed protocol for interaction mating is presented.

Conclusions:

The updated yeast two-hybrid system offers enhanced resources for researchers.
The new interaction mating protocol provides a valuable alternative for yeast two-hybrid screening.
This advancement facilitates more efficient and comprehensive studies of protein interactions.