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DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: Jul 7, 2026

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

Preparation of yeast DNA.

¹Boston College, Chestnut Hill, Massachusetts, USA.

Current Protocols in Molecular Biology

|February 12, 2008

Summary

This study details two "smash and grab" DNA isolation protocols for yeast. These methods efficiently yield both plasmid and chromosomal DNA for molecular biology applications like bacterial transformation and PCR.

Area of Science:

Molecular Biology
Yeast Genetics

Background:

Isolation of plasmid and chromosomal DNA is crucial for yeast molecular studies.
Plasmid DNA is essential for E. coli transformation.
Chromosomal DNA is required for Southern hybridization, PCR, and cloning.

Purpose of the Study:

To present efficient protocols for isolating both plasmid and chromosomal yeast DNA.
To provide DNA suitable for various downstream molecular applications.
To offer versatile methods applicable to both Saccharomyces cerevisiae and Schizosaccharomyces pombe.

Main Methods:

Two variations of the "smash and grab" protocol were employed.
The protocols were optimized for DNA yield and purity.
Applicability was tested across different yeast species.

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Last Updated: Jul 7, 2026

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

Yeast As a Chassis for Developing Functional Assays to Study Human P53

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Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

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Published on: February 27, 2026

Main Results:

The "smash and grab" protocols successfully isolated both plasmid and chromosomal DNA.
The isolated DNA was suitable for bacterial transformation.
The DNA was also effective for Southern hybridization and PCR.

Conclusions:

The presented "smash and grab" protocols are effective for yeast DNA isolation.
These methods provide high-quality DNA for diverse molecular biology techniques.
The protocols are robust and applicable to common laboratory yeast strains.