Related Experiment Video
Updated: Jul 7, 2026
05:41
Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish
Published on: January 28, 2020
Measurement of in situ hybridization
1Molecular Histology Laboratory, Inc., Gaithersburg, Maryland, USA.
Current Protocols in Molecular Biology
|February 12, 2008
Summary
Molecular probes enable precise localization of gene expression and foreign DNA within tissues. This technique, using phosphor storage imaging, quantifies nucleic acid amounts and locations for accurate analysis.
Area of Science:
- Molecular biology
- Genomics
- Biotechnology
Background:
- Gene expression analysis and pathogen detection are crucial in biological research and diagnostics.
- Accurate spatial localization of nucleic acids within tissues is essential for understanding biological processes and disease states.
Purpose of the Study:
- To describe a method for the geometric and functional localization of nucleic acids in tissues.
- To enable the estimation of target nucleic acid sequence amounts and locations.
Main Methods:
- Utilizing hybridization of labeled specific molecular probes to target nucleic acids (DNA or RNA) within tissue samples.
- Employing phosphor storage imaging techniques for signal detection and quantification.
- Implementing molecular controls to ensure accuracy and reliability of results.
Main Results:
- Demonstrated the capability to achieve precise geometric and functional localization of gene expression or foreign genome sequences.
- Successfully estimated the amounts and locations of target nucleic acid sequences within the analyzed tissues.
Conclusions:
- Hybridization with labeled probes offers a powerful tool for in situ nucleic acid analysis.
- The described method provides a reliable approach for quantifying and localizing specific nucleic acid sequences in biological tissues.
Related Concept Videos
02:31In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
02:07FISH - Fluorescent In-situ Hybridization
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...