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Updated: Jul 7, 2026

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
Published on: February 25, 2021
Analysis of disaccharides and tetrasaccharides released from glycosaminoglycans
1University of Illinois, Urbana, Illinois, USA.
Abstract:
Glycosaminoglycans (GAGs) are converted to disaccharides by various methods. This unit describes separation of individual disaccharides by paper chromatography or paper electrophoresis or high-performance liquid chromatography (HPLC). Lyase-released disaccharides can also be monitored by UV absorbance. Support protocols describe mild conditions for reduction of alkali-labile disaccharides obtained by cleavage of GAGs with lyases, scintillation counting of samples obtained from HPLC separation of radiolabeled saccharides, and calculations for the quantitation of disaccharides.
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