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Updated: Jul 7, 2026

Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
Published on: October 29, 2009
Purification of oligonucleotides using denaturing polyacrylamide gel electrophoresis
1University of Texas, Austin, Texas, USA.
Abstract:
Cloning vectors derived from filamentous phage are extremely useful because they allow cloned DNA to be isolated as either single- or double-stranded DNA. This unit contains protocols for preparing both forms of DNA and for characterizing inserts in M13-derived vectors. A protocol is also presented for preparing single-stranded DNA from plasmids using superinfection with helper phage. This method is advantageous because it allows cloned DNA to be maintained in the form of a plasmid while permitting single-stranded DNA to be isolated for DNA sequencing.
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