Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA ligases.

S Tabor1

  • 1Harvard Medical School, Boston, Massachusetts, USA.

Current Protocols in Molecular Biology
|February 12, 2008
PubMed
Summary
This summary is machine-generated.

DNA ligases join DNA fragments by forming phosphodiester bonds. T4 DNA ligase uses ATP and can ligate blunt ends, while E. coli ligase uses NAD and typically requires cohesive ends.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Asymmetric split H-shape nanoantennas for molecular sensing.

Biomedical optics express·2017
Same author

The impact of feeding diets of high or low energy concentration on carcass measurements and the weight of primal and subprimal lean cuts.

Asian-Australasian journal of animal sciences·2014
Same author

Enzymatic labeling of DNA.

Current protocols in human genetics·2008
Same author

Expression using the T7 RNA polymerase/promoter system.

Current protocols in molecular biology·2008
Same author

Phosphatases and kinases.

Current protocols in molecular biology·2008
Same author

Exonucleases.

Current protocols in molecular biology·2008
Same journal

Nondenaturing Polyacrylamide Gel Electrophoresis: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Purification and Concentration of DNA from Aqueous Solutions: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Expression of Proteins Using Semliki Forest Virus Vectors: Protein Expression.

Current protocols in molecular biology·2021
Same journal

Methylation and Uracil Interference Assays for Analysis of Protein-DNA Interactions: DNA-Protein Interactions.

Current protocols in molecular biology·2021
Same journal

Separation of Double- and Single-Stranded Nucleic Acids Using Hydroxylapatite Chromatography: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Pulsed-Field Gel Electrophoresis: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
See all related articles

Area of Science:

  • Molecular Biology
  • Enzymology

Background:

  • DNA ligases are crucial enzymes that repair and join DNA strands.
  • They form phosphodiester bonds between DNA termini, essential for DNA replication and repair.

Purpose of the Study:

  • To describe the reaction conditions and applications of two key DNA ligases: E. coli ligase and T4 ligase.
  • To highlight the differences in energy sources and substrate specificities between these enzymes.

Main Methods:

  • Enzymatic assays to assess DNA ligation activity.
  • Comparative analysis of E. coli ligase and T4 ligase under various reaction conditions.

Main Results:

  • Both E. coli ligase and T4 ligase can repair nicks in duplex DNA.
  • T4 DNA ligase utilizes ATP as an energy source and efficiently ligates blunt ends.

Related Experiment Videos

  • E. coli ligase uses NAD as an energy source and is less efficient with blunt ends, primarily ligating cohesive ends.

    • Conclusions:

    • Understanding the distinct properties of DNA ligases is vital for selecting the appropriate enzyme in molecular biology research.
    • T4 DNA ligase is preferred for blunt-end ligations, while E. coli ligase is suitable for cohesive-end ligations.