Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification

Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification

Published on: May 18, 2020

AFLP-based transcript profiling.

Pieter Vos¹, Patrick Stanssens

¹Keygene N.V., The Netherlands.

Current Protocols in Molecular Biology

|February 12, 2008

Summary

This study introduces a new method for quantifying gene transcripts using Amplified Fragment Length Polymorphism (AFLP)-fingerprinting of complementary DNA (cDNA). This technique reliably identifies differentially expressed messenger RNAs (mRNAs).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Post-traumatic parkinsonism: The intricate twist between trauma, inflammation and neurodegeneration. A narrative review.

Journal of the neurological sciences·2024

Same author

Successful epilepsy surgery in two cases with multiple sclerosis.

Epileptic disorders : international epilepsy journal with videotape·2023

Same author

Effect of digital-enabled multidisciplinary therapy conferences on efficiency and quality of the decision making in prostate cancer care.

BMJ health & care informatics·2022

Same author

Long-Term Stability of Blood Serum Biomarkers in Traumatic Brain Injury: A Feasibility Study.

Frontiers in neurology·2022

Same author

Prediction of Neurocognitive Outcome after Moderate-Severe Traumatic Brain Injury Using Serum Neuron-Specific Enolase and S100 biomarkers.

Journal of medicine and life·2020

Same author

Deep Learning Regression for Prostate Cancer Detection and Grading in Bi-Parametric MRI.

IEEE transactions on bio-medical engineering·2020

Area of Science:

Molecular Biology
Gene Expression Analysis

Background:

Differential display is a common technique for identifying gene expression differences.
Quantifying transcript levels and identifying differentially expressed mRNAs requires efficient and reliable methods.

Purpose of the Study:

To present an alternative method to differential display for transcript quantification.
To enable the identification of differentially expressed mRNAs using AFLP-fingerprinting.

Main Methods:

Isolation of poly(A)+ RNA from total RNA.
Synthesis of double-stranded cDNA.
Preparation of template fragments via restriction enzyme digestion and adaptor ligation.
Selective amplification of specific fragment subsets.
Electrophoretic analysis of amplification products on denaturing polyacrylamide gels.

Main Results:

Successful implementation of AFLP-fingerprinting for transcript analysis.
Generation of reliable transcript profiles.
Identification of differentially expressed mRNAs.

Conclusions:

AFLP-fingerprinting of double-stranded cDNA provides an effective alternative to differential display.
The described protocol is a reliable and efficient tool for quantifying transcripts and identifying differentially expressed mRNAs.

More Related Videos

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Published on: April 30, 2011

Related Experiment Videos

Last Updated: Jul 7, 2026

Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification

Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification

Published on: May 18, 2020

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Published on: April 30, 2011