Related Experiment Video

Updated: Jul 7, 2026

Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays
07:35

Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays

Published on: April 7, 2015

S. pombe strain maintenance and media

Susan L Forsburg1

  • 1Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California, USA.

Current Protocols in Molecular Biology
|February 12, 2008
PubMed

Insights

Fission yeast (S. pombe) cultivation methods are similar to budding yeast, but optimal media differs. Maintaining sterile techniques is crucial for preventing contamination in yeast cultures.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Fission yeast (Schizosaccharomyces pombe) is a model organism.
  • Culturing techniques are fundamental for yeast research.
  • Understanding optimal growth conditions is essential for experimental success.

Purpose of the Study:

  • To outline standard culture methods for fission yeast.
  • To highlight differences in media requirements between fission and budding yeast.
  • To emphasize the importance of sterile techniques.

Main Methods:

  • Standard microbiological culture techniques.
  • Media preparation and optimization.
  • Sterile handling procedures.

Main Results:

  • Fission yeast can be cultured using methods similar to budding yeast (Saccharomyces cerevisiae).
  • Specific media formulations are optimal for S. pombe, though S. cerevisiae media can be substituted.
  • Strict adherence to sterile techniques prevents microbial and mold contamination.

Conclusions:

  • Fission yeast cultivation is achievable with established yeast culture protocols.
  • Media optimization for S. pombe is recommended for best results.
  • Sterile technique is paramount for reliable fission yeast experiments.

More Related Videos

Fission Yeast as a Platform for Antibacterial Drug Screens Targeting Bacterial Cytoskeleton Proteins05:57

Fission Yeast as a Platform for Antibacterial Drug Screens Targeting Bacterial Cytoskeleton Proteins

Published on: April 26, 2024

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes03:53

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes

Published on: April 19, 2024

Related Concept Videos

Microbial Growth Media01:27

Microbial Growth Media

Microbial growth media are essential tools in microbiology, providing the nutrients and conditions necessary to cultivate and study microorganisms. These media are categorized by their composition, consistency, and functional roles, enabling researchers to investigate microbial physiology, behavior, and interactions.Types and Consistencies of Growth MediaGrowth media can be solid, liquid, or semisolid. Solid media, often agar-based, allow visible colony growth for isolation and enumeration.
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Designing Growth Media for Bioreactors01:30

Designing Growth Media for Bioreactors

Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
Bacterial Phylum Tenericutes01:24

Bacterial Phylum Tenericutes

The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...