Related Experiment Video
Updated: Jul 7, 2026

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization
Published on: September 8, 2023
Imaging objects hidden in scattering media with fluorescence polarization preservation of contrast agents
Abstract:
A method for fluorescence polarization difference imaging is demonstrated for enhancing the image quality of a luminous object embedded in a random medium. The polarization preservation of light propagating in the scattering medium leads to partially polarized light emission by a contrast-agent dye located inside the object. Subtraction of the images of the luminous object detected at two orthogonal polarization directions improves the image resolution compared with a conventional optical imaging approach.
Related Concept Videos
Super-resolution Fluorescence Microscopy
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Immunofluorescence Microscopy
The...
Confocal Fluorescence Microscopy
