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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
Published on: January 28, 2019
Australian Rotavirus Surveillance Program: annual report, 2006-07
Carl D Kirkwood1, David Cannan, Nada Bogdanovic-Sakran
1National Rotavirus Reference Centre, Murdoch Childrens Research Institute, Royal Children's Hospital Parkville, Victoria. carl.kirkwood@mcri.edu.au
Insights
Rotavirus surveillance in Australia identified G1 as the dominant serotype in hospitalized children with acute gastroenteritis. G9 and G3 were also prevalent, while G2 and G4 were less common.
Area of Science:
- Virology
- Public Health
- Epidemiology
Background:
- Rotavirus is a leading cause of severe gastroenteritis in young children globally.
- Effective rotavirus surveillance is crucial for understanding circulating strains and informing vaccination strategies.
Purpose of the Study:
- To characterize the rotavirus serotypes causing acute gastroenteritis in hospitalized children in Australia.
- To provide data on the prevalence of different rotavirus genotypes during the surveillance period.
Main Methods:
- Laboratory-based surveillance of rotavirus strains from hospitalized children across Australia.
- Analysis of 1002 faecal samples using monoclonal antibody immunoassays, RT-PCR, and PAGE.
- Determination of G and P genotypes of identified rotavirus strains.
Main Results:
- Serotype G1 was the most common (36.7%), followed by G9 (31.1%) and G3 (23.3%).
- Serotype G2 accounted for less than 5% of strains; no G4 strains were detected.
- The P[8] genotype was predominant among G1, G3, and G9 strains.
- Rare genotypes G8 and G12 were identified in a small number of cases.
Conclusions:
- Serotype G1, G9, and G3 were the primary rotavirus serotypes responsible for childhood hospitalizations due to gastroenteritis in Australia during 2006-2007.
- The findings highlight the importance of ongoing rotavirus surveillance to monitor evolving epidemiology.
Abstract:
The National Rotavirus Reference Centre, together with collaborating laboratories Australia-wide, conducts a laboratory based rotavirus surveillance program. This report describes the serotypes of rotavirus strains responsible for the hospitalisation of children with acute gastroenteritis during the period 1 July 2006 to 30 June 2007. One thousand and two faecal samples from across Australia were examined using a combined approach of monoclonal antibody immunoassays, reverse transcription-polymerase chain reaction and polyacrylamide gel analysis. Serotype G1 was the dominant serotype nationally, representing 36.7% of all strains, followed by serotype G9 (31.1%), and serotype G3 (23.3%). Serotype G2 represented less than 5% of strains, while no serotype G4 strains were identified. All G1, G3 and G9 strains assayed for P genotype contained the P[8] genotype, bar one G1 strain, which possessed a P[6]. Uncommon rotavirus genotypes, G8 (n = 1) and G12 (n = 2) were identified in children with acute gastroenteritis during this study period.
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