Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genome mapping and protein coding region identification using bacteriophage Mu.

E A Groisman1, N Pagratis, M J Casadaban

  • 1Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637.

Gene
|March 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The novel CaMKII inhibitor GS-680 reduces diastolic SR Ca leak and prevents CaMKII-dependent pro-arrhythmic activity.

Journal of molecular and cellular cardiology·2018
Same author

Medical, demographical and social aspects of syphilis: the case of infected sex workers in Greece during Interwar.

Giornale italiano di dermatologia e venereologia : organo ufficiale, Societa italiana di dermatologia e sifilografia·2014
Same author

A Mg2+-responding RNA that controls the expression of a Mg2+ transporter.

Cold Spring Harbor symposia on quantitative biology·2007
Same author

Type III secretion system genes in clinical Aeromonas isolates.

Journal of clinical microbiology·2004
Same author

Pathogenicity islands of Shigella.

Current topics in microbiology and immunology·2002
Same author

Salmonella enterica serovar Typhimurium response involved in attenuation of pathogen intracellular proliferation.

Infection and immunity·2001

A novel DNA cloning system uses bacteriophage Mu transposition and bacteriophage T7 promoters for efficient gene cloning and expression. This method facilitates bacterial gene characterization and offers an alternative to traditional recombinant DNA techniques.

Area of Science:

  • Molecular Biology
  • Bacteriophage Biology
  • Genetics

Background:

  • Bacteriophage Mu is a versatile transposon.
  • Recombinant DNA technologies are standard for gene cloning.
  • A need exists for alternative gene cloning methods.

Purpose of the Study:

  • To develop a DNA-cloning and gene-expressing system using bacteriophage Mu.
  • To combine Mu transposition with a bacteriophage T7 promoter.
  • To demonstrate the system's utility in characterizing bacterial DNA.

Main Methods:

  • Developed a bacteriophage Mu-based vector (38 kb capacity).
  • Integrated bacteriophage T7 promoter for gene expression.
  • Utilized in vivo transcription and host translation for gene identification.

Related Experiment Videos

  • Applied to characterize a 35-kb region of Escherichia coli K-12.

    • Main Results:

    • Successfully cloned and expressed genes using the novel system.
    • Demonstrated applicability to Escherichia coli K-12.
    • Validated the system's potential for other Enterobacteriaceae and Mu-like phage hosts.

    Conclusions:

    • The developed system offers an efficient alternative for bacterial gene cloning and expression.
    • This bacteriophage Mu and T7 promoter-based system is adaptable for various bacterial species.
    • The technology has broad implications for microbial genomics and synthetic biology.