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Related Experiment Videos

Bacteriophage P22 accessory recombination function.

A R Poteete1, A C Fenton, A V Semerjian

  • 1Department of Molecular Genetics and Microbiology, University of Massachusetts, Worcester 01655.

Virology
|May 1, 1991
PubMed
Summary
This summary is machine-generated.

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The accessory recombination function (arf) gene in bacteriophage P22 is crucial for homologous recombination and plaque formation. Mutants lacking functional arf show defects, but arf expression can restore these functions.

Area of Science:

  • Molecular Biology
  • Virology
  • Genetics

Background:

  • The accessory recombination function (arf) gene is situated upstream of the essential recombination function (erf) gene in bacteriophage P22.
  • Understanding arf's role is key to deciphering P22's recombination mechanisms.

Purpose of the Study:

  • To investigate the function of the accessory recombination function (arf) gene in bacteriophage P22.
  • To characterize the effects of arf mutations on phage recombination and growth.

Main Methods:

  • Construction and analysis of three mutant alleles of the arf gene: an out-of-frame deletion, an in-frame deletion, and an amber mutation.
  • Assessing phage growth and homologous recombination in various bacterial hosts (wild-type, recA, recB, recA recB, and suppressor strains).

Related Experiment Videos

  • Complementation assays using plasmids expressing the arf gene.

    • Main Results:

    • Deletion mutants of arf exhibited partial defects in homologous recombination and plaque formation, with more severe phenotypes in recB and recA recB hosts.
    • The amber mutant displayed similar growth defects in nonsuppressing hosts but not in suppressor hosts.
    • Expression of arf from plasmids complemented the growth defects of arf- phages and stimulated erf-mediated recombination.
    • Arf also showed a minor stimulation of recA-recBCD-mediated homologous recombination in phage lambda.

    Conclusions:

    • The accessory recombination function (arf) gene plays a significant role in bacteriophage P22's homologous recombination and replication.
    • Arf function is partially dependent on host recombination machinery, particularly RecB.
    • Arf can complement its own defects and influence other recombination pathways.