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Updated: Jul 7, 2026
Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
Published on: May 16, 2020
[Effects of millimeter wave on gene expression in human keratinocytes]
Qing Chen1, De-qiang Lu, Huai Jiang
1Bioelectromagnetics Laboratory of Zhejiang Province, College of Medicine, Zhejiang University, Hangzhou 310058, China.
Objective:
To explore the effect of millimeter wave exposure at low power density on gene expression in human keratinocytes (HaCaT).
Methods:
HaCaT keratinocytes were exposed to 30.16 GHz millimeter wave with power densities of 1.0 or 3.5 mW/cm2 for 30 min per day. Gene expression profiles were obtained using the Affymetrix human genome U95A GeneChip. Reverse-transcription polymerase chain reaction (RT-PCR) was performed to confirm the differential expression of genes obtained from Genechip analysis.
Result:
PAR-2 and ERGIC-53 genes in HaCaT cells were up-regulated by 3.5 mW/cm2 millimeter wave exposure for 4 times. ERGIC-53 gene was also up-regulated by 1.0 mW/cm2 millimeter wave exposure for 4 times. However, no significant change for PAR-2 expression was found after the same exposure.
Conclusion:
Millimeter wave exposure could affect gene expression in human keratinocytes, which might be related to the intensity and the times of exposure.
