Related Experiment Video
Updated: Jul 7, 2026

Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain
Published on: August 28, 2012
A method for the prevention of thrombin-induced degradation of recombinant proteins
Dakshinamurthy Rajalingam1, Karuppanan Muthusamy Kathir, Koteshwara Ananthamurthy
1Department of Chemistry & Biochemistry, University of Arkansas, Fayetteville, AR 72701, USA.
Abstract:
A new strategy to prevent degradation of recombinant proteins caused by non-specific cleavage by thrombin is described. We demonstrate that degradation due to non-specific cleavage of recombinant protein mediated by thrombin can be completely prevented by separation of thrombin from the recombinant protein on spin columns packed with heparin-sepharose. This method is generally applicable to all recombinant proteins that require the thrombin for the cleavage of affinity tags for purification. To our knowledge, this is the first report of an efficient and reliable method for the separation of residual thrombin from purified recombinant proteins.
Related Concept Videos
Anticoagulant Drugs: Low-Molecular-Weight Heparins
Venous Thrombosis III: Interprofessional Care
Tagging and Fusion Proteins
