Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 7, 2026

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
13:19

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

Efficient identification of a novel cancer/testis antigen for immunotherapy using three-step microarray analysis.

Takeshi Yokoe1, Fumiaki Tanaka, Koshi Mimori

  • 1Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan.

Cancer Research
|February 19, 2008
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Haplotype analysis of spinocerebellar ataxia type 36 suggests a shared permissive core haplotype across populations.

Journal of human genetics·2026
Same author

Two scripts, two pathways: dorsal-ventral biases in post-stroke kana-kanji agraphia.

Brain : a journal of neurology·2026
Same author

Leptomeningeal Carcinomatosis Arising 29 Years After Gastrectomy: A Report of a Rare Autopsy Case.

Cureus·2026
Same author

Real-world outcomes and early discontinuation of foslevodopa/foscarbidopa in Parkinson's disease.

Journal of neurology·2026
Same author

Asthma-like bronchodilator responsiveness in patients with neuronal intranuclear inclusion disease.

Scientific reports·2026
Same author

Complete remission of intracranial lymphocytic vasculitis following resection: illustrative case.

Journal of neurosurgery. Case lessons·2026

Researchers identified serine/threonine kinase 31 (STK31) as a novel cancer/testis antigen. STK31 is highly expressed in colorectal, gastric, and esophageal cancers, showing potential for gastrointestinal cancer immunotherapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • Advanced molecular biology tools aid cancer diagnosis and treatment.
  • Cancer/testis (CT) antigens are targets for cancer immunotherapy.
  • Identifying novel CT antigens is crucial for developing new cancer therapies.

Purpose of the Study:

  • To develop a new methodology for identifying novel cancer/testis (CT) antigens.
  • To identify and characterize a novel CT antigen expressed in colorectal cancer.
  • To evaluate the potential of the identified antigen as a target for cancer immunotherapy.

Main Methods:

  • Combined laser microdissection and cDNA microarray to analyze gene expression profiles.
  • Compared gene expression in colorectal cancer cells with normal colon epithelium and normal testis.

More Related Videos

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Related Experiment Videos

Last Updated: Jul 7, 2026

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
13:19

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

  • Utilized reverse transcription-polymerase chain reaction (RT-PCR) to validate gene expression levels.

    • Main Results:

    • Identified 38 candidate CT antigens using the novel methodology.
    • Discovered serine/threonine kinase 31 (STK31) as a novel CT antigen.
    • STK31 showed significantly higher expression in colorectal cancer tissues compared to normal tissues (P < 0.0001).
    • STK31 was also frequently expressed in gastric and esophageal cancers.
    • STK31 peptide successfully elicited specific cytotoxic T lymphocytes (CTLs) that lysed target cells.

    Conclusions:

    • The developed methodology effectively facilitates the identification of novel CT antigens.
    • STK31 is a promising candidate for cancer immunotherapy, particularly against gastrointestinal cancers.
    • STK31-specific CTLs demonstrate potential for targeted cancer treatment.