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Evolution toward small molecule inhibitor resistance affects native enzyme function and stability, generating

Ronan M Kelly1, Hans Leemhuis, Linda Gätjen

  • 1Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Carbohydrate Bioprocessing, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.

The Journal of Biological Chemistry
|February 20, 2008
PubMed
Summary

Developing resistance to small molecule inhibitors like acarbose can significantly impair enzyme function and stability. Evolving cyclodextrin glucanotransferase (CGTase) for acarbose insensitivity revealed substantial costs to catalytic efficiency and thermostability.

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