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Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
Chemosensitization of fungal pathogens to antimicrobial agents using benzo analogs
Jong H Kim1, Noreen Mahoney, Kathleen L Chan
1Plant Mycotoxin Research Unit, Western Regional Research Center, USDA-ARS, Albany, CA 94710, USA.
Abstract:
Activities of conventional antifungal agents, fludioxonil, strobilurin and antimycin A, which target the oxidative and osmotic stress response systems, were elevated by coapplication of certain benzo analogs (aldehydes and acids). Fungal tolerance to 2,3-dihydroxybenzaldehyde or 2,3-dihydroxybenzoic acid was found to rely upon mitochondrial superoxide dismutase (SOD2) or glutathione reductase (GLR1), genes regulated by the HOG1 signaling pathway, respectively. Thus, certain benzo analogs can be effective at targeting cellular oxidative stress response systems. The ability of these compounds to chemosensitize fungi for improved control with conventional antifungal agents is discussed.
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