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Purification of NAD glycohydrolase from Neurospora crassa conidia by a polyclonal immunoadsorbent
M Pace1, D Agnellini, G Lippoli
1Dipartimento di Scienze e Tecnologie Biomediche, University of Milan, Italy.
Journal of Chromatography
|February 22, 1991
Abstract:
NAD glycohydrolase from Neurospora crassa conidia was purified by affinity chromatography on a column of polyclonal antibodies bound to an agarose matrix. The procedure was easy, non-denaturating and suitable for repetitive use of the gel. The enzyme obtained appeared homogeneous by sodiumdodecyl sulphate-polyacrylamide gel electrophoresis.