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Autofluorescent proteins for flow cytometry.

Charles G Bailey¹, John E J Rasko

¹Gene and Stem Cell Therapy Program, Centenary Institute of Cancer Medicine and Cell Biology, Australia.

Methods in Molecular Biology (Clifton, N.J.)

|February 22, 2008

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Autofluorescent proteins like enhanced green fluorescent protein (eGFP), orange fluorescent protein (cOFP), and DsREDExpress are versatile tools for flow cytometry. Their spectral properties enable sophisticated cellular analysis and high-speed, multiparametric studies.

Area of Science:

Biochemistry
Cell Biology
Biotechnology

Background:

Autofluorescent proteins offer unique spectral properties and versatility for flow cytometry.
Advancements in multilaser flow cytometers enable high-speed, multiparametric analysis of biological samples.
Efficient gene transfer and expression are crucial for optimal fluorescent protein utilization in flow cytometry.

Purpose of the Study:

To highlight the utility of autofluorescent proteins in flow cytometry.
To demonstrate the compatibility of enhanced green fluorescent protein (eGFP) with other fluorescent protein variants.
To showcase Cerianthus sp. orange fluorescent protein (cOFP) and DsREDExpress for flow cytometric applications.

Main Methods:

Utilizing autofluorescent proteins for transient or stable expression analysis.

Related Experiment Videos

Employing multilaser flow cytometers for high-speed, multiparametric analysis.

Assessing the spectral properties and compatibility of eGFP, cOFP, and DsREDExpress.

Main Results:

Autofluorescent proteins are well-suited for flow cytometric applications.
eGFP can be successfully combined with other variants like cOFP and DsREDExpress.
cOFP and DsREDExpress do not require special filters for excitation and detection with eGFP.

Conclusions:

Autofluorescent proteins, including eGFP, cOFP, and DsREDExpress, are valuable for flow cytometry due to their spectral properties and ease of use.
The combination of these proteins facilitates sophisticated cellular analysis and experimental designs.
Efficient gene transfer and expression are key to leveraging these tools for advanced biological research.